FastQCFastQC Report
Thu 30 Jan 2020
Control_R1_S14_L008_R1_001_x.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameControl_R1_S14_L008_R1_001_x.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500000
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATC468650.045.3442231
CTAATCT207.8699016E-443.9798932
AGTTTCG207.8776595E-443.97109639
TGACCGG207.8776595E-443.97109644
AGTACGG207.8776595E-443.97109644
TGTGGGA254.456473E-543.9710929
TTTTGGA502.7284841E-1143.9710928
CCTATAG254.456473E-543.97109244
CGGAAAT207.88154E-443.966728
ACACGTA207.88154E-443.966733
CGCGGAA454.820322E-1043.966728
CCGGAAT1250.043.966727
CGGGGAT351.4524994E-743.966728
GGGACTA207.88154E-443.966729
CGGGACA650.043.966728
GCGGAGG207.88154E-443.966729
CGCGTAT207.88154E-443.966728
GATAGCT207.88154E-443.966729
CGTTGGG302.5380232E-643.96669828
CGTACTA254.4591052E-543.96669828
Produced by FastQC (version 0.11.5)