FastQCFastQC Report
Thu 30 Jan 2020
Control_R2_S15_L008_R1_001_x.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameControl_R2_S15_L008_R1_001_x.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500000
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAATCTTGTGGAAAGGACGAAACACCGCTGCCTAGCTGCGACGCCATG6700.134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATC479500.045.3596461
TCGATCG254.4561722E-543.971644
TCGTCTA254.4561722E-543.971641
TTTCGCA207.877217E-443.97159641
AGCCAGG207.877217E-443.97159644
GGATATG207.877217E-443.97159639
TCATCGG302.5360805E-643.97159644
TACCGCA207.877217E-443.97159638
CGGAATA351.4523721E-743.967228
CGGAAAT207.881098E-443.967228
CCGGGGT950.043.967227
CCGGGCT1500.043.967227
CGTATGA551.8189894E-1243.967228
GTGGGCT207.881098E-443.967229
CGTTAGT254.4588043E-543.967228
CATAGGA254.4588043E-543.967231
GGGAACG254.4588043E-543.967229
CGAATAG254.4588043E-543.967228
CGGTGGG207.881098E-443.967228
CGTAAAG408.35098E-943.967228
Produced by FastQC (version 0.11.5)