FastQCFastQC Report
Thu 30 Jan 2020
ToxA_R1_98_S2_L008_R1_001_x.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameToxA_R1_98_S2_L008_R1_001_x.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500000
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAATCTTGTGGAAAGGACGAAACACCGTCCGCCTGAAGTACTGCACCG25410.5082No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGGCAAGTACCTGTCAGACCGCG17690.3538No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGTCTTGAACTAGCTGCCACCCG17570.35140000000000005No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGCTTCAACCAAGTGACGCTACG16660.3332No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGATATCCATGTAGCGACAAGAG16490.32980000000000004No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGGCGAGGACGACGAGTTCTACG14810.29619999999999996No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGGCCCTCTCCGAAGCCGCTCCG13450.269No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGAGCAAAGGTTATTGTTCAGTG13080.2616No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGATCCATACAACTGCTCCGCCG12460.24919999999999998No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGTGACCGTAGGGATGGCCACTG11750.23500000000000001No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGTCACCTGGGCGTGCGCAGCGG11090.2218No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGCGCACACCTGCTTATATACGT9120.1824No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGGACAAGCTACGTCTCCACACG8170.1634No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGGGCCGCCCATGGATGTAGTCG7310.1462No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGCAACGGGTTCTCCCGGCTACG6620.13240000000000002No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGCACTCACAGGCTGCCCAGCCG6610.1322No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGGAAGAAGTCGGCCTCCTCTAG6610.1322No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGTCACCTGGTTACCGCTCTCCG6410.12819999999999998No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGTGCCGTTAGCATTCGATCCTG6290.1258No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGGACCATGAAGCTGCTGCCGTG6240.1248No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGAAACATCGAGTCACTTTGCCG5950.11900000000000001No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGAAGGGCTTGAGTGTCAGATGT5880.1176No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGACAGTATTGGCATCGATTCCG5710.1142No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGGGTGTCACCACAGACCTCGTT5550.11100000000000002No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGCATGTCGTCCACCAGCACCGG5430.10859999999999999No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGTGCAAGGACCTGGTATGAATG5050.101No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGACTTAAGCTGTGACTTGCTGG5050.101No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGCTAATGTAAGCAGTCCCCCGT5030.1006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATC467600.044.887721
GCGAGAA207.860036E-443.99109641
TATTTCG408.310963E-943.99109644
ATGGGTA207.860036E-443.99109636
ATCCTAA207.860036E-443.99109641
AGGTAGT302.5283534E-643.99109635
GGTACAC254.4445245E-543.99109634
AATTTAG302.5283534E-643.99109636
GGTAACC207.860036E-443.99109630
CAACGGA254.4445245E-543.99109630
CACGGAA254.4445245E-543.99109632
GCGGTGC254.4445245E-543.99109630
TTAGCAG254.4445245E-543.99109632
TGGAATT207.860036E-443.99109641
GACCGTT207.860036E-443.99109642
ACACATT502.7284841E-1143.99109643
ATAGCCT207.860036E-443.99109641
GGATCCG207.860036E-443.99109632
TATATTA254.4445245E-543.99109634
AATGGTT207.860036E-443.99109644
Produced by FastQC (version 0.11.5)