FastQCFastQC Report
Thu 30 Jan 2020
ToxA_R2_S2_L005_R1_001_x.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameToxA_R2_S2_L005_R1_001_x.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500000
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAATCTTGTGGAAAGGACGAAACACCGTCCGCCTGAAGTACTGCACCG21540.4308No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGGCAAGTACCTGTCAGACCGCG15890.31779999999999997No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGCTTCAACCAAGTGACGCTACG13730.2746No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGGCGAGGACGACGAGTTCTACG11710.2342No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGTCTTGAACTAGCTGCCACCCG8960.1792No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGTCACCTGGGCGTGCGCAGCGG8610.1722No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGCGCACACCTGCTTATATACGT8140.1628No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGATATCCATGTAGCGACAAGAG8060.16119999999999998No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGTGACCGTAGGGATGGCCACTG7530.15059999999999998No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGAGCAAAGGTTATTGTTCAGTG6700.134No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGGCCCTCTCCGAAGCCGCTCCG6480.1296No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGGGCCGCCCATGGATGTAGTCG6180.12359999999999999No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGATCCATACAACTGCTCCGCCG5990.1198No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGCACTCACAGGCTGCCCAGCCG5810.11620000000000001No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGGAAGAAGTCGGCCTCCTCTAG5680.1136No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGAAGGGCTTGAGTGTCAGATGT5260.1052No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGCAACGGGTTCTCCCGGCTACG5220.1044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATC476000.044.4624251
CTTAAGG207.8628527E-443.987944
AAGTAAA254.446433E-543.987932
CGGAAAA502.7284841E-1143.987928
GATACGG207.8628527E-443.987936
CGAACCG254.446433E-543.987928
CGTATAG254.446433E-543.987928
CGAGTGG408.3164196E-943.987928
ACGTTTA207.8628527E-443.987930
CAACGGA254.446433E-543.987930
CGCGGGT302.5296195E-643.987928
CTATATT207.8628527E-443.987933
GTTTAAA207.8628527E-443.987929
GTGGGTA207.8628527E-443.987929
TTAGAGT207.8628527E-443.987938
TTAGAAC351.447006E-743.987936
ATAGCGG207.8628527E-443.987944
AAGACCG207.8628527E-443.987944
GGATCGG254.446433E-543.987944
ATTAAGC254.446433E-543.987931
Produced by FastQC (version 0.11.5)