FastQCFastQC Report
Thu 30 Jan 2020
ToxB_R1_98_S4_L008_R1_001_x.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameToxB_R1_98_S4_L008_R1_001_x.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500000
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTCTTGTGGAAAGGACGAAACACCGGCAAGCTGTAGTGCGCGCGCGTT114232.2846No Hit
GACTCTTGTGGAAAGGACGAAACACCGACCTGTTGTACATCTCAGTGTTT62321.2464No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTATTCATAAAAGTGCAGTAGTT61661.2331999999999999No Hit
GACTCTTGTGGAAAGGACGAAACACCGATCCTGGACGAGTTTATAATGTT45160.9032No Hit
GACTCTTGTGGAAAGGACGAAACACCGAGCCTAATGTCCTGAAACACGTT44350.8869999999999999No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTCCCGACTGAACATCTATAGTT41820.8364No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTAAGTACCTGGCAGGTCAAGTT41740.8348No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCAGCATCAGCACTGCACTTGTT38270.7654No Hit
GACTCTTGTGGAAAGGACGAAACACCGCGACGTCGATGTGCCCACACGTT34980.6996No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCAGGTCATCTATCGTGTGTGTT34100.6819999999999999No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTGTGTCCAGTCCCGCTTCGGTT31940.6387999999999999No Hit
GACTCTTGTGGAAAGGACGAAACACCGACTCCCGCACACGCTCTGTTGTT29530.5906No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCTGCTGTAGGCTCATTTGTGTT28900.5780000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGACATGACACAGAAGAAAGCGGTT28410.5682No Hit
GACTCTTGTGGAAAGGACGAAACACCGACCGAGCTTCAATGACCTTGTTT28270.5654No Hit
GACTCTTGTGGAAAGGACGAAACACCGTACGTACATGGGGGTCCGTGTTT28130.5626No Hit
GACTCTTGTGGAAAGGACGAAACACCGTACTGCTGTGGCCCACGCCGGTT27840.5568No Hit
GACTCTTGTGGAAAGGACGAAACACCGAATCGTGGCCCTCACTGCTAGTT27770.5554No Hit
GACTCTTGTGGAAAGGACGAAACACCGCAAGATGACCAGCCGCCTGCGTT27320.5464No Hit
GACTCTTGTGGAAAGGACGAAACACCGTGTCAGCTATATGCATTGAAGTT27210.5442No Hit
GACTCTTGTGGAAAGGACGAAACACCGTGGCACTCACGTCTTACACTGTT27080.5416No Hit
GACTCTTGTGGAAAGGACGAAACACCGGTCCGCCGGCTGCTATGCCTGTT26950.539No Hit
GACTCTTGTGGAAAGGACGAAACACCGGGAGTGTGTGAAGTACTGCGGTT26640.5328No Hit
GACTCTTGTGGAAAGGACGAAACACCGTGCTCTCTCACTCTCCCTAAGTT25560.5112No Hit
GACTCTTGTGGAAAGGACGAAACACCGCGTCCACTCAAATGTCATTGTTT25140.5028No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCTCGGAGCCCGCTATGTCAGTT25070.5014000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGGGGTTCCAGTGTACCGCCACGTT25020.5004No Hit
GACTCTTGTGGAAAGGACGAAACACCGACTGGAGTGTAGAGGCATCTGTT24160.4832No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTCCATGAGTTTCCGATTGAGTT23940.47879999999999995No Hit
GACTCTTGTGGAAAGGACGAAACACCGTGAGGCACCGCCCAAAGCCTGTT23510.47019999999999995No Hit
GACTCTTGTGGAAAGGACGAAACACCGGTTTAAGGAATATGCTGTCCGTT22850.457No Hit
GACTCTTGTGGAAAGGACGAAACACCGCACGTATGACCCTGAAACCTGTT22250.445No Hit
GACTCTTGTGGAAAGGACGAAACACCGAAGAGGCCCACCTCCTCATGTTT21420.42839999999999995No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCCACTGGCCGTTGAAGTAGGTT21370.4274No Hit
GACTCTTGTGGAAAGGACGAAACACCGGACCAACTAATTGAAGTCCTGTT20380.40759999999999996No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCGTCCAGACCGTCCGCAGTGTT19740.39480000000000004No Hit
GACTCTTGTGGAAAGGACGAAACACCGCGTAATTCTGATCAGCATCTGTT19620.3924No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCCTGCTTCTTGTACATCATGTT19570.39139999999999997No Hit
GACTCTTGTGGAAAGGACGAAACACCGGTGGTCCTTGTTAGCTGTCTGTT19080.3816No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCTGATAGCCATTCCAAAGTGTT17320.3464No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCTGAGTCTCGTGTGTCATGGTT17110.3422No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCGTCTGCTCATGTGCTCAAGTT16860.3372No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCGCCAATCAGTGTACCCGCGTT16630.3326No Hit
GACTCTTGTGGAAAGGACGAAACACCGGCGCTCCGTATACCGCACCAGTT16120.32239999999999996No Hit
GACTCTTGTGGAAAGGACGAAACACCGACCAAGAGAATATCTTCCGAGTT16040.3208No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTGACCTGGAACCACACCGTGTT14840.2968No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTGTTTGCATCTGTACACAAGTT13600.272No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCAGCCGCCGCACACACGCCGTT13350.267No Hit
GACTCTTGTGGAAAGGACGAAACACCGACTCTTCGATAGCCTCGGGCGTT12870.25739999999999996No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTCTCAACTTTGGGAAGAATGTT12820.25639999999999996No Hit
GACTCTTGTGGAAAGGACGAAACACCGTAATGCGAAACAACACCTCTGTT12650.253No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCACCTCCGGCTGTACTTCGGTT11760.2352No Hit
GACTCTTGTGGAAAGGACGAAACACCGGAAGAGGCAGGGTAGTGTAAGTT11730.2346No Hit
GACTCTTGTGGAAAGGACGAAACACCGATGCAACAAGATCCGATACTGTT11490.2298No Hit
GACTCTTGTGGAAAGGACGAAACACCGATTGTTTGCTGCCTATGCGCGTT11330.22659999999999997No Hit
GACTCTTGTGGAAAGGACGAAACACCGCGCCTTCTCCTCACTCGCCGTTT11290.2258No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCGTCGTTGAGGCCGCACTGGTT11220.2244No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCTCTTGGTGGGGCCCACGAGTT11200.22399999999999998No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTGCTTCCTGCTAGATTACTGTT11160.2232No Hit
GACTCTTGTGGAAAGGACGAAACACCGATGGCCGTCACCTATACTTTGTT10770.2154No Hit
GACTCTTGTGGAAAGGACGAAACACCGGACCCGAGGAATTCTCCACCGCG10490.20980000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGGTTCTTACCAAGTAATCATCGTT10100.202No Hit
GACTCTTGTGGAAAGGACGAAACACCGGTCAAAGGTCGAATCCCCAGTTT10070.20140000000000002No Hit
GACTCTTGTGGAAAGGACGAAACACCGAGTGTCTTCCCGCCAGCGCTGTT9920.19840000000000002No Hit
GACTCTTGTGGAAAGGACGAAACACCGCGCGCAGGGCTTCCGCCGACGTT9650.193No Hit
GACTCTTGTGGAAAGGACGAAACACCGGTGAGATGTATGTATTCTGTTTT9580.1916No Hit
GACTCTTGTGGAAAGGACGAAACACCGGCCACTCAGGCATCCAATAGGTT9290.1858No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCGTACCGCCTCCGTTTGTCGTT9230.1846No Hit
GACTCTTGTGGAAAGGACGAAACACCGAGGCCCATCTCCTGGCCATCGTT9120.1824No Hit
GACTCTTGTGGAAAGGACGAAACACCGGCATGTCGATACTTCCAGTCGTT8980.1796No Hit
GACTCTTGTGGAAAGGACGAAACACCGAGGAAAAAAACCTTTAACTAGTT8940.17880000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGTGATGAAGATGCTGCAGTCCGTT8930.1786No Hit
GACTCTTGTGGAAAGGACGAAACACCGCAGCTTCACGCCCAGACCCTGTT8920.1784No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTCAGTTGCAGTCGACACCCGTT8840.1768No Hit
GACTCTTGTGGAAAGGACGAAACACCGGTCCGGGAAGCAGCGCACCCGTT8810.1762No Hit
GACTCTTGTGGAAAGGACGAAACACCGACACCCACCATTCAGCACATGTT8740.1748No Hit
GACTCTTGTGGAAAGGACGAAACACCGGGCACAGCAGCTTAATTGACGTT8710.1742No Hit
GACTCTTGTGGAAAGGACGAAACACCGATCCGAAGCATGCACGTTTGGTT8610.1722No Hit
GACTCTTGTGGAAAGGACGAAACACCGGCTGTGATTGCCGTCCGAAATTA8580.1716No Hit
GACTCTTGTGGAAAGGACGAAACACCGACCTTTAAGGTTAGGGTCTCGTT8470.1694No Hit
GACTCTTGTGGAAAGGACGAAACACCGCATCACACGGGCCTCATCGGTTT8430.1686No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCCACGGGTAATCTGCAGGGGTT8110.16219999999999998No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTCCTCATTGACAATTCCGCGTT8090.1618No Hit
GACTCTTGTGGAAAGGACGAAACACCGTACTACGTTCAGCTAAATTAGTT8080.1616No Hit
GACTCTTGTGGAAAGGACGAAACACCACCTCGAGCGCCGAAGCACTCGTT8050.161No Hit
GACTCTTGTGGAAAGGACGAAACACCGCAACGGGTTCTCCCGGCTACGTT8020.1604No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTGTTGTTGTAGCTTCGAAAGTT7900.158No Hit
GACTCTTGTGGAAAGGACGAAACACCGTGTCTGTGTCTTCAGCGCCAGTT7880.1576No Hit
GACTCTTGTGGAAAGGACGAAACACCGAGACCAGACAATTTCGTGTTGTT7870.1574No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTAGGACTGCGTGATCTTTGGTT7790.1558No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCGTATGTATAACATCAAGAGTT7770.1554No Hit
GACTCTTGTGGAAAGGACGAAACACCGACCCACCCCGTAGTTGTTCAGTT7700.154No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTGGGCCTACCGTGTCTAGGGTT7620.1524No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCCGCAAAAAGTTGTGCCTGGTT7530.15059999999999998No Hit
GACTCTTGTGGAAAGGACGAAACACCGACCCTTCCAGATCCGCATCCGTT7530.15059999999999998No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTCCTGCCAGGCTGAGATTGGTT7520.15039999999999998No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCCTGCCACCATGACGGAACGTT7470.1494No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCTTTTTTTGTTGCTTGTCTGTT7440.1488No Hit
GACTCTTGTGGAAAGGACGAAACACCGTATTATGATCATCAGTGCACGTT7350.147No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTGGATTTCTCCTGAGATTTGTT7290.1458No Hit
GACTCTTGTGGAAAGGACGAAACACCGCGCTCACCGCGGGTCTCCTCGTT7040.1408No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTGGGCTTCAGCGTGCGCGGGTT7000.13999999999999999No Hit
GACTCTTGTGGAAAGGACGAAACACCGAGGGTATGGCGGCAGCGAGAGTT6900.13799999999999998No Hit
GACTCTTGTGGAAAGGACGAAACACCGGACCAAATCATCACTAATCAGTT6820.1364No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCAACTTTCTATTATCACTGTTT6810.1362No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTTGAGCACACCATCCATGTGTT6680.1336No Hit
GACTCTTGTGGAAAGGACGAAACACCGTATGACTAGAATCGTCTCCCGTT6670.1334No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTGAATTCCTCAATACGTGTGTT6630.1326No Hit
GACTCTTGTGGAAAGGACGAAACACCGCAACTGTCTCACCAATCATAGTT6540.1308No Hit
GACTCTTGTGGAAAGGACGAAACACCAACCAACAAGGTTGAGACGCAGTT6480.1296No Hit
GACTCTTGTGGAAAGGACGAAACACCGCGAACCTGACGCGCCACCTGGTT6420.1284No Hit
GACTCTTGTGGAAAGGACGAAACACCGGCGGAAGGCATCATTATCGAGTT6320.12639999999999998No Hit
GACTCTTGTGGAAAGGACGAAACACCGGGGCGAAATGGATATGGGTAGTT6310.1262No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTGTACCTGTAATGAGGTGAGTT6170.12340000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGGGCCGACTCACCCCTTTCGCGTT6140.12279999999999999No Hit
GACTCTTGTGGAAAGGACGAAACACCGACCACAGGGGCCTTCAAAGCGTT6100.122No Hit
GACTCTTGTGGAAAGGACGAAACACCGAGTATCATAGACTCCGAAGTGTT6070.12140000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCATCGAGCCCAGAACTTCTCACGTT6020.1204No Hit
GACTCTTGTGGAAAGGACGAAACACCGGAATCGGCACTTGATCCCATGTT5970.11939999999999999No Hit
GACTCTTGTGGAAAGGACGAAACACCGCACCTGGCTCAGCATTTAACGTT5920.11839999999999999No Hit
GACTCTTGTGGAAAGGACGAAACACCGGTAATACCAGCTATGTTCAAGTT5900.11800000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGGAATCTATCTGTAGCAAAAGGTT5860.11720000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCATGACATCCACAAATGATGTT5820.1164No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTGGAGTTGAGACAGACGACGTT5800.116No Hit
GACTCTTGTGGAAAGGACGAAACACCGTGTACCTTACAGACTCCTGTGTT5730.11460000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGGAACAATGAGCAGTTCTGATGTT5720.1144No Hit
GACTCTTGTGGAAAGGACGAAACACCGGAGAAGTGTATTCGAATCCTGTT5610.1122No Hit
GACTCTTGTGGAAAGGACGAAACACCGGCCTATCGGCATTCCCCCGGGTT5540.11080000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCTCTCACCGACTCTGTGTCGTT5500.11No Hit
GACTCTTGTGGAAAGGACGAAACACCGTGGATGGGACTTAGAGCCCTGTT5500.11No Hit
GACTCTTGTGGAAAGGACGAAACACCGATAGGTCAAACACATCTTCTGTT5490.10980000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGATGCCCGTTCACTACCAGCAGTT5490.10980000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCCGGAGACCCTGCACACCTGTT5300.106No Hit
GACTCTTGTGGAAAGGACGAAACACCGGATCTGCACGTCGATGAATCGTT5290.10579999999999999No Hit
GACTCTTGTGGAAAGGACGAAACACCGCACAAACCACGTTACACGAAGTT5280.10560000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTGGAAGTAAGTCCAATCACGTT5210.1042No Hit
GACTCTTGTGGAAAGGACGAAACACCACCGCCAACAGAATTCATACGGTT5200.104No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCCGGTTAAATTTAAGAGAAGTT5180.1036No Hit
GACTCTTGTGGAAAGGACGAAACACCGTGTCCCCGGAACCCTCCGAGGTT5170.10339999999999999No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCGCTTTGGACTGCCTTCGCGTT5150.10300000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTAGGGGATGACTTTGACTTGTT5110.10219999999999999No Hit
GACTCTTGTGGAAAGGACGAAACACCGGCCGAACCATCACTTCTGCGGTT5100.10200000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCAGCGCTCCCGCAGCAGTTTTA5080.1016No Hit
GACTCTTGTGGAAAGGACGAAACACCGGCAACTGAGATGTCTCCAATGTT5040.1008No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCTCAGACTGGTTTCATCCTGTT5020.10039999999999999No Hit
GACTCTTGTGGAAAGGACGAAACACCGGTTTACCTGTGGTGCCAACTGTT5020.10039999999999999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCTT468450.044.6099361
GTCATTT408.305506E-943.99436
TTCTATT502.7284841E-1143.99434
TTGATAT254.4427936E-543.99441
AGACTAG207.8574824E-443.99435
TAATAAC408.305506E-943.99430
ACAGTTG551.8189894E-1243.99442
ACAGTTA408.305506E-943.99431
GATACGT551.8189894E-1243.99443
TCGCATC207.8574824E-443.99441
TTGGCCC207.8574824E-443.99436
ACTGCGG2950.043.99442
ATCTAGG254.4427936E-543.99430
GTACAAT207.8574824E-443.99432
AGTCAAG408.305506E-943.99442
ACGTTTT254.4427936E-543.99444
CTATCTA408.305506E-943.99434
ACGCAAT207.8574824E-443.99436
ATGGATA551.8189894E-1243.99435
ATAATGG207.8574824E-443.99442
Produced by FastQC (version 0.11.5)