FastQCFastQC Report
Thu 30 Jan 2020
ToxB_R2_S4_L005_R1_001_x.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameToxB_R2_S4_L005_R1_001_x.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500000
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTCTTGTGGAAAGGACGAAACACCGGCAAGCTGTAGTGCGCGCGCGTT118232.3646000000000003No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTATTCATAAAAGTGCAGTAGTT65731.3146No Hit
GACTCTTGTGGAAAGGACGAAACACCGACCTGTTGTACATCTCAGTGTTT64371.2874No Hit
GACTCTTGTGGAAAGGACGAAACACCGATCCTGGACGAGTTTATAATGTT46940.9388000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGAGCCTAATGTCCTGAAACACGTT46870.9374No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTCCCGACTGAACATCTATAGTT44800.8959999999999999No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTAAGTACCTGGCAGGTCAAGTT43980.8796No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCAGCATCAGCACTGCACTTGTT39150.783No Hit
GACTCTTGTGGAAAGGACGAAACACCGCGACGTCGATGTGCCCACACGTT36550.731No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCAGGTCATCTATCGTGTGTGTT35420.7084No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTGTGTCCAGTCCCGCTTCGGTT34860.6972No Hit
GACTCTTGTGGAAAGGACGAAACACCGACTCCCGCACACGCTCTGTTGTT30940.6188No Hit
GACTCTTGTGGAAAGGACGAAACACCGCAAGATGACCAGCCGCCTGCGTT30520.6104No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCTGCTGTAGGCTCATTTGTGTT30110.6022No Hit
GACTCTTGTGGAAAGGACGAAACACCGACCGAGCTTCAATGACCTTGTTT29510.5902000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGAATCGTGGCCCTCACTGCTAGTT29330.5866No Hit
GACTCTTGTGGAAAGGACGAAACACCGGTCCGCCGGCTGCTATGCCTGTT29100.582No Hit
GACTCTTGTGGAAAGGACGAAACACCGTACTGCTGTGGCCCACGCCGGTT29100.582No Hit
GACTCTTGTGGAAAGGACGAAACACCGTACGTACATGGGGGTCCGTGTTT29030.5806No Hit
GACTCTTGTGGAAAGGACGAAACACCGTGTCAGCTATATGCATTGAAGTT28750.575No Hit
GACTCTTGTGGAAAGGACGAAACACCGACATGACACAGAAGAAAGCGGTT28550.571No Hit
GACTCTTGTGGAAAGGACGAAACACCGTGGCACTCACGTCTTACACTGTT28360.5672No Hit
GACTCTTGTGGAAAGGACGAAACACCGTGCTCTCTCACTCTCCCTAAGTT27790.5558No Hit
GACTCTTGTGGAAAGGACGAAACACCGGGAGTGTGTGAAGTACTGCGGTT27250.545No Hit
GACTCTTGTGGAAAGGACGAAACACCGGGGTTCCAGTGTACCGCCACGTT27140.5428No Hit
GACTCTTGTGGAAAGGACGAAACACCGCGTCCACTCAAATGTCATTGTTT26870.5374No Hit
GACTCTTGTGGAAAGGACGAAACACCGACTGGAGTGTAGAGGCATCTGTT25900.518No Hit
GACTCTTGTGGAAAGGACGAAACACCGTGAGGCACCGCCCAAAGCCTGTT25880.5176000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCTCGGAGCCCGCTATGTCAGTT25630.5126000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTCCATGAGTTTCCGATTGAGTT25280.5055999999999999No Hit
GACTCTTGTGGAAAGGACGAAACACCGCACGTATGACCCTGAAACCTGTT24560.49119999999999997No Hit
GACTCTTGTGGAAAGGACGAAACACCGGTTTAAGGAATATGCTGTCCGTT23830.4766No Hit
GACTCTTGTGGAAAGGACGAAACACCGAAGAGGCCCACCTCCTCATGTTT22200.44400000000000006No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCCACTGGCCGTTGAAGTAGGTT21540.4308No Hit
GACTCTTGTGGAAAGGACGAAACACCGGACCAACTAATTGAAGTCCTGTT21430.4286No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCGTCCAGACCGTCCGCAGTGTT21420.42839999999999995No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCCTGCTTCTTGTACATCATGTT21010.4202No Hit
GACTCTTGTGGAAAGGACGAAACACCGCGTAATTCTGATCAGCATCTGTT20220.4044No Hit
GACTCTTGTGGAAAGGACGAAACACCGGTGGTCCTTGTTAGCTGTCTGTT19470.38939999999999997No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCTGATAGCCATTCCAAAGTGTT19020.3804No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCGTCTGCTCATGTGCTCAAGTT18900.378No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCGCCAATCAGTGTACCCGCGTT18080.3616No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCTGAGTCTCGTGTGTCATGGTT18070.3614No Hit
GACTCTTGTGGAAAGGACGAAACACCGACCAAGAGAATATCTTCCGAGTT17090.3418No Hit
GACTCTTGTGGAAAGGACGAAACACCGGCGCTCCGTATACCGCACCAGTT16550.331No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTGTTTGCATCTGTACACAAGTT15030.3006No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTGACCTGGAACCACACCGTGTT14960.29919999999999997No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCAGCCGCCGCACACACGCCGTT13490.2698No Hit
GACTCTTGTGGAAAGGACGAAACACCGTAATGCGAAACAACACCTCTGTT13290.26580000000000004No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCACCTCCGGCTGTACTTCGGTT12910.2582No Hit
GACTCTTGTGGAAAGGACGAAACACCGACTCTTCGATAGCCTCGGGCGTT12860.2572No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTCTCAACTTTGGGAAGAATGTT12760.2552No Hit
GACTCTTGTGGAAAGGACGAAACACCGATGCAACAAGATCCGATACTGTT12210.24420000000000003No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCGTCGTTGAGGCCGCACTGGTT12200.244No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCTCTTGGTGGGGCCCACGAGTT11770.23540000000000003No Hit
GACTCTTGTGGAAAGGACGAAACACCGGAAGAGGCAGGGTAGTGTAAGTT11750.23500000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTGCTTCCTGCTAGATTACTGTT11490.2298No Hit
GACTCTTGTGGAAAGGACGAAACACCGATGGCCGTCACCTATACTTTGTT11470.2294No Hit
GACTCTTGTGGAAAGGACGAAACACCGGTCAAAGGTCGAATCCCCAGTTT11390.2278No Hit
GACTCTTGTGGAAAGGACGAAACACCGATTGTTTGCTGCCTATGCGCGTT11210.2242No Hit
GACTCTTGTGGAAAGGACGAAACACCGCGCCTTCTCCTCACTCGCCGTTT11210.2242No Hit
GACTCTTGTGGAAAGGACGAAACACCGGACCCGAGGAATTCTCCACCGCG11020.22039999999999998No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTCCTGCCAGGCTGAGATTGGTT10790.21580000000000002No Hit
GACTCTTGTGGAAAGGACGAAACACCGAGTGTCTTCCCGCCAGCGCTGTT10530.21059999999999998No Hit
GACTCTTGTGGAAAGGACGAAACACCGGTTCTTACCAAGTAATCATCGTT10520.2104No Hit
GACTCTTGTGGAAAGGACGAAACACCGGTGAGATGTATGTATTCTGTTTT9970.19940000000000002No Hit
GACTCTTGTGGAAAGGACGAAACACCGAGGAAAAAAACCTTTAACTAGTT9890.1978No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCGTACCGCCTCCGTTTGTCGTT9800.196No Hit
GACTCTTGTGGAAAGGACGAAACACCGGCCACTCAGGCATCCAATAGGTT9670.1934No Hit
GACTCTTGTGGAAAGGACGAAACACCGCGCGCAGGGCTTCCGCCGACGTT9650.193No Hit
GACTCTTGTGGAAAGGACGAAACACCGTGATGAAGATGCTGCAGTCCGTT9620.1924No Hit
GACTCTTGTGGAAAGGACGAAACACCGAGGCCCATCTCCTGGCCATCGTT9550.191No Hit
GACTCTTGTGGAAAGGACGAAACACCGCAGCTTCACGCCCAGACCCTGTT9500.19No Hit
GACTCTTGTGGAAAGGACGAAACACCGGCTGTGATTGCCGTCCGAAATTA9240.1848No Hit
GACTCTTGTGGAAAGGACGAAACACCGACACCCACCATTCAGCACATGTT9220.18439999999999998No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTCCTCATTGACAATTCCGCGTT9210.1842No Hit
GACTCTTGTGGAAAGGACGAAACACCGCAACGGGTTCTCCCGGCTACGTT9180.18359999999999999No Hit
GACTCTTGTGGAAAGGACGAAACACCGGGCACAGCAGCTTAATTGACGTT9140.1828No Hit
GACTCTTGTGGAAAGGACGAAACACCGATCCGAAGCATGCACGTTTGGTT9090.1818No Hit
GACTCTTGTGGAAAGGACGAAACACCGTGTCTGTGTCTTCAGCGCCAGTT9070.1814No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCCGCAAAAAGTTGTGCCTGGTT8850.17700000000000002No Hit
GACTCTTGTGGAAAGGACGAAACACCGGTCCGGGAAGCAGCGCACCCGTT8770.1754No Hit
GACTCTTGTGGAAAGGACGAAACACCGGCATGTCGATACTTCCAGTCGTT8770.1754No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCCACGGGTAATCTGCAGGGGTT8660.1732No Hit
GACTCTTGTGGAAAGGACGAAACACCGCATCACACGGGCCTCATCGGTTT8630.1726No Hit
GACTCTTGTGGAAAGGACGAAACACCGAGACCAGACAATTTCGTGTTGTT8560.1712No Hit
GACTCTTGTGGAAAGGACGAAACACCGTACTACGTTCAGCTAAATTAGTT8550.17099999999999999No Hit
GACTCTTGTGGAAAGGACGAAACACCGACCTTTAAGGTTAGGGTCTCGTT8520.1704No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTGTTGTTGTAGCTTCGAAAGTT8450.169No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTCAGTTGCAGTCGACACCCGTT8340.1668No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTGGGCCTACCGTGTCTAGGGTT8290.1658No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCGTATGTATAACATCAAGAGTT8280.1656No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCTTTTTTTGTTGCTTGTCTGTT8240.1648No Hit
GACTCTTGTGGAAAGGACGAAACACCGACCCACCCCGTAGTTGTTCAGTT7900.158No Hit
GACTCTTGTGGAAAGGACGAAACACCACCTCGAGCGCCGAAGCACTCGTT7870.1574No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCCTGCCACCATGACGGAACGTT7860.1572No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTGGATTTCTCCTGAGATTTGTT7830.1566No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTAGGACTGCGTGATCTTTGGTT7740.1548No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCAACTTTCTATTATCACTGTTT7730.15460000000000002No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTGAATTCCTCAATACGTGTGTT7490.1498No Hit
GACTCTTGTGGAAAGGACGAAACACCGCGCTCACCGCGGGTCTCCTCGTT7320.1464No Hit
GACTCTTGTGGAAAGGACGAAACACCGTATGACTAGAATCGTCTCCCGTT7300.146No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTTGAGCACACCATCCATGTGTT7270.1454No Hit
GACTCTTGTGGAAAGGACGAAACACCGACCCTTCCAGATCCGCATCCGTT7200.14400000000000002No Hit
GACTCTTGTGGAAAGGACGAAACACCGTATTATGATCATCAGTGCACGTT7200.14400000000000002No Hit
GACTCTTGTGGAAAGGACGAAACACCGGACCAAATCATCACTAATCAGTT7170.1434No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTGGGCTTCAGCGTGCGCGGGTT7130.1426No Hit
GACTCTTGTGGAAAGGACGAAACACCGCAACTGTCTCACCAATCATAGTT7120.1424No Hit
GACTCTTGTGGAAAGGACGAAACACCGAGGGTATGGCGGCAGCGAGAGTT7050.14100000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGGGGCGAAATGGATATGGGTAGTT6870.1374No Hit
GACTCTTGTGGAAAGGACGAAACACCGGTAATACCAGCTATGTTCAAGTT6870.1374No Hit
GACTCTTGTGGAAAGGACGAAACACCGACCACAGGGGCCTTCAAAGCGTT6610.1322No Hit
GACTCTTGTGGAAAGGACGAAACACCAACCAACAAGGTTGAGACGCAGTT6580.1316No Hit
GACTCTTGTGGAAAGGACGAAACACCGCACCTGGCTCAGCATTTAACGTT6550.131No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTGGAGTTGAGACAGACGACGTT6540.1308No Hit
GACTCTTGTGGAAAGGACGAAACACCGATAGGTCAAACACATCTTCTGTT6410.12819999999999998No Hit
GACTCTTGTGGAAAGGACGAAACACCGGGCCGACTCACCCCTTTCGCGTT6400.128No Hit
GACTCTTGTGGAAAGGACGAAACACCGCGAACCTGACGCGCCACCTGGTT6380.1276No Hit
GACTCTTGTGGAAAGGACGAAACACCGGAATCTATCTGTAGCAAAAGGTT6320.12639999999999998No Hit
GACTCTTGTGGAAAGGACGAAACACCGATGCCCGTTCACTACCAGCAGTT6320.12639999999999998No Hit
GACTCTTGTGGAAAGGACGAAACACCGTGGATGGGACTTAGAGCCCTGTT6200.124No Hit
GACTCTTGTGGAAAGGACGAAACACCATCGAGCCCAGAACTTCTCACGTT6180.12359999999999999No Hit
GACTCTTGTGGAAAGGACGAAACACCGGCGGAAGGCATCATTATCGAGTT6160.1232No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTGTACCTGTAATGAGGTGAGTT6050.121No Hit
GACTCTTGTGGAAAGGACGAAACACCGGAACAATGAGCAGTTCTGATGTT6020.1204No Hit
GACTCTTGTGGAAAGGACGAAACACCGGAATCGGCACTTGATCCCATGTT6000.12No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCATGACATCCACAAATGATGTT5930.1186No Hit
GACTCTTGTGGAAAGGACGAAACACCGCTGGAAGTAAGTCCAATCACGTT5860.11720000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCTCTCACCGACTCTGTGTCGTT5840.1168No Hit
GACTCTTGTGGAAAGGACGAAACACCGTGTACCTTACAGACTCCTGTGTT5820.1164No Hit
GACTCTTGTGGAAAGGACGAAACACCGTGATTCAAACCTTGATCCTGGTT5810.11620000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGTCCGGAGACCCTGCACACCTGTT5760.1152No Hit
GACTCTTGTGGAAAGGACGAAACACCGGCCGAACCATCACTTCTGCGGTT5740.1148No Hit
GACTCTTGTGGAAAGGACGAAACACCGGAGAAGTGTATTCGAATCCTGTT5690.11379999999999998No Hit
GACTCTTGTGGAAAGGACGAAACACCGCACAAACCACGTTACACGAAGTT5670.1134No Hit
GACTCTTGTGGAAAGGACGAAACACCGGCCTATCGGCATTCCCCCGGGTT5620.1124No Hit
GACTCTTGTGGAAAGGACGAAACACCGAGTATCATAGACTCCGAAGTGTT5580.1116No Hit
GACTCTTGTGGAAAGGACGAAACACCACCGCCAACAGAATTCATACGGTT5560.1112No Hit
GACTCTTGTGGAAAGGACGAAACACCGGATCTGCACGTCGATGAATCGTT5500.11No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCAGCGCTCCCGCAGCAGTTTTA5420.1084No Hit
GACTCTTGTGGAAAGGACGAAACACCGTTAGGGGATGACTTTGACTTGTT5370.10740000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCGCTTTGGACTGCCTTCGCGTT5360.1072No Hit
GACTCTTGTGGAAAGGACGAAACACCGCCCGGTTAAATTTAAGAGAAGTT5320.10640000000000001No Hit
GACTCTTGTGGAAAGGACGAAACACCGGCAACTGAGATGTCTCCAATGTT5310.1062No Hit
GACTCTTGTGGAAAGGACGAAACACCGGTTTACCTGTGGTGCCAACTGTT5260.1052No Hit
GACTCTTGTGGAAAGGACGAAACACCGGGCTTCCCCATGTTCAAAAGGTT5140.1028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTTTT454.292815E-1044.4995461
GACTCTT476650.044.2614821
TGTGGGA351.4458965E-743.99227
GTGTAAA351.4458965E-743.99228
TGTAAAG351.4458965E-743.99229
GTAAAGG351.4458965E-743.992210
GGGGAAA408.309144E-943.9921958
TGTGGCA302.5279187E-643.9921957
TGGAACG302.5279187E-643.9921959
GGCAAGG302.5279187E-643.99219510
GCGGAAA207.85907E-443.9921958
CGGACAA551.8189894E-1243.98780426
CTATGAT1100.043.98780436
TGATACT1050.043.98780439
CACGGTT1050.043.98780444
GTGATAT551.8189894E-1243.98780427
TGACGGA1100.043.98780439
CATTATC1100.043.98780439
TATTGAC551.8189894E-1243.98780431
TGGTTAA551.8189894E-1243.98780441
Produced by FastQC (version 0.11.5)