##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	Control_R2_S15_L008_R1_001_x.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	500000
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.61767	32.0	32.0	32.0	32.0	32.0
2	31.6811	32.0	32.0	32.0	32.0	32.0
3	35.14864	37.0	32.0	37.0	32.0	37.0
4	35.80451	37.0	37.0	37.0	32.0	37.0
5	36.1592	37.0	37.0	37.0	37.0	37.0
6	39.657242	41.0	41.0	41.0	37.0	41.0
7	39.89413	41.0	41.0	41.0	37.0	41.0
8	39.879178	41.0	41.0	41.0	37.0	41.0
9	40.036922	41.0	41.0	41.0	37.0	41.0
10	39.498924	41.0	41.0	41.0	37.0	41.0
11	39.594872	41.0	41.0	41.0	37.0	41.0
12	39.60236	41.0	41.0	41.0	37.0	41.0
13	39.555686	41.0	41.0	41.0	37.0	41.0
14	39.681814	41.0	41.0	41.0	37.0	41.0
15	39.975716	41.0	41.0	41.0	37.0	41.0
16	40.011492	41.0	41.0	41.0	37.0	41.0
17	39.646586	41.0	41.0	41.0	37.0	41.0
18	39.307806	41.0	41.0	41.0	37.0	41.0
19	39.377546	41.0	41.0	41.0	37.0	41.0
20	39.934092	41.0	41.0	41.0	37.0	41.0
21	39.6255	41.0	41.0	41.0	37.0	41.0
22	39.50177	41.0	41.0	41.0	37.0	41.0
23	39.599824	41.0	41.0	41.0	37.0	41.0
24	39.707324	41.0	41.0	41.0	37.0	41.0
25	39.929736	41.0	41.0	41.0	37.0	41.0
26	39.954684	41.0	41.0	41.0	37.0	41.0
27	40.02935	41.0	41.0	41.0	37.0	41.0
28	39.6063	41.0	41.0	41.0	37.0	41.0
29	39.169442	41.0	41.0	41.0	37.0	41.0
30	38.47313	41.0	41.0	41.0	32.0	41.0
31	38.346832	41.0	41.0	41.0	32.0	41.0
32	38.242232	41.0	41.0	41.0	32.0	41.0
33	38.283524	41.0	41.0	41.0	32.0	41.0
34	38.310664	41.0	41.0	41.0	32.0	41.0
35	38.34492	41.0	41.0	41.0	32.0	41.0
36	38.322888	41.0	41.0	41.0	32.0	41.0
37	38.323348	41.0	41.0	41.0	32.0	41.0
38	38.305926	41.0	41.0	41.0	32.0	41.0
39	38.28549	41.0	41.0	41.0	32.0	41.0
40	38.269596	41.0	41.0	41.0	32.0	41.0
41	38.246882	41.0	41.0	41.0	32.0	41.0
42	38.24456	41.0	41.0	41.0	32.0	41.0
43	38.26743	41.0	41.0	41.0	32.0	41.0
44	38.198958	41.0	41.0	41.0	32.0	41.0
45	38.193414	41.0	41.0	41.0	32.0	41.0
46	38.13647	41.0	41.0	41.0	32.0	41.0
47	38.179144	41.0	41.0	41.0	32.0	41.0
48	38.379394	41.0	41.0	41.0	32.0	41.0
49	38.517506	41.0	41.0	41.0	32.0	41.0
50	38.940938	41.0	41.0	41.0	32.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.16025668708716623
1101	2	0.008770216947567633
1101	3	0.03539079628971109
1101	4	0.0295543996790002
1101	5	-0.002465958654966016
1101	6	0.11046296517312015
1101	7	-0.0028071930297528525
1101	8	0.0031178103697016013
1101	9	0.01766137498307785
1101	10	-0.046518452074082006
1101	11	-0.04389822368167273
1101	12	0.08416141354088325
1101	13	0.11071769209993931
1101	14	-0.023928376742333057
1101	15	-0.011649391567608802
1101	16	-0.04694196079657331
1101	17	0.0596122041201852
1101	18	0.07244496987521387
1101	19	0.03256830969606028
1101	20	0.06931201022224798
1101	21	0.11510603961791332
1101	22	0.11221527146812349
1101	23	0.01745832342772502
1101	24	-0.009879275751529804
1101	25	0.11422381250307723
1101	26	-0.0885274804322691
1101	27	-0.024380455395416334
1101	28	0.04969419442716827
1101	29	0.04525032669778284
1101	30	0.006686560043263512
1101	31	0.10102495876759576
1101	32	-0.0077125764074139624
1101	33	0.05824675304131688
1101	34	-0.03718298950867904
1101	35	-0.05346306159820102
1101	36	-0.08828645999639662
1101	37	0.03124076538806264
1101	38	0.02917331727167749
1101	39	0.07862271820223299
1101	40	-0.01270823000576371
1101	41	5.373925462279772E-4
1101	42	0.04331251933796665
1101	43	0.030596618022357802
1101	44	0.018359826533831836
1101	45	0.0293197242342913
1101	46	-0.040298556986748224
1101	47	-0.05425697619853764
1101	48	-0.131688075708162
1101	49	-0.04919957644160888
1101	50	0.07595058830195711
1102	1	0.10030223798239746
1102	2	0.01093771796231735
1102	3	-0.02584313687224693
1102	4	-0.09350382428708315
1102	5	-0.048522423725096075
1102	6	-0.030359205935880595
1102	7	0.0034111806679888446
1102	8	-0.003571676844472904
1102	9	-0.05912291221306276
1102	10	0.009301418940218298
1102	11	0.10642415037686703
1102	12	-0.09688761004636604
1102	13	-0.043287268888647645
1102	14	0.020958287218334704
1102	15	-0.055234923520885104
1102	16	0.03182158044728567
1102	17	0.046082145039321176
1102	18	8.507787418565727E-4
1102	19	-0.00461475078289908
1102	20	0.047990222462360066
1102	21	0.05249349282676974
1102	22	-0.007149000394051086
1102	23	0.04832559443300255
1102	24	-0.015349893169108952
1102	25	0.03846257135393216
1102	26	-0.02620131798244074
1102	27	0.08278853391590957
1102	28	0.12567615446866398
1102	29	0.11056120028277405
1102	30	0.09584301158342612
1102	31	0.033521598825323906
1102	32	0.08464759136420241
1102	33	-0.11561145288480645
1102	34	-0.019257223937941603
1102	35	-0.030688876778832253
1102	36	0.12458297580390365
1102	37	0.04131545081628474
1102	38	0.06678735235939115
1102	39	0.039117554264109344
1102	40	0.09067669760890595
1102	41	0.04252710976987828
1102	42	0.07668409738333537
1102	43	0.10787231368825445
1102	44	0.11796799863503082
1102	45	0.052975161026786566
1102	46	-0.07952148577400919
1102	47	0.0989329394646532
1102	48	0.0361213321302003
1102	49	0.014701654785554297
1102	50	0.05751672729983426
1103	1	0.10981397444840368
1103	2	0.038775155187156685
1103	3	0.03931869583057335
1103	4	0.08625635800976994
1103	5	0.03603367016663128
1103	6	0.07598578643154497
1103	7	0.07734627059962662
1103	8	0.03366006581608616
1103	9	0.040741161590617025
1103	10	-0.0384370824203728
1103	11	0.016156766911898046
1103	12	0.01872487640304854
1103	13	0.007176747394176175
1103	14	-0.06134661754040849
1103	15	0.1277491697513824
1103	16	0.059285840087561326
1103	17	0.13024010614637405
1103	18	-0.04859511847281084
1103	19	-0.01865160837454738
1103	20	0.02893568827063575
1103	21	-0.014613518080068388
1103	22	0.026013274776850892
1103	23	0.007938434856882282
1103	24	0.033091512994474215
1103	25	2.388152423407064E-4
1103	26	0.04558921762517798
1103	27	0.014093239564012094
1103	28	0.06834388305873773
1103	29	-0.16055350956877845
1103	30	-0.01650830807763981
1103	31	0.0449046213248252
1103	32	0.011987163497558129
1103	33	0.13587625756407817
1103	34	0.05760797815935348
1103	35	0.03957142719674778
1103	36	-0.1252776025018889
1103	37	0.004720419732905157
1103	38	0.0426243471161456
1103	39	0.10965528573022709
1103	40	0.1047859429795821
1103	41	0.19236990085857286
1103	42	0.1110555493423675
1103	43	0.01166408820652265
1103	44	-0.009658469805927439
1103	45	-0.13595083618566406
1103	46	-0.029394718667084874
1103	47	0.02929598453003024
1103	48	0.1263946446675348
1103	49	0.02454471135509806
1103	50	0.01089846664188343
1104	1	-0.08570814372335178
1104	2	-0.007741366461889498
1104	3	-0.06198010632067508
1104	4	0.041516841998273435
1104	5	0.026558892442089643
1104	6	-0.06860856112619729
1104	7	0.004935197805359337
1104	8	0.012469849978529624
1104	9	0.0022740639262792683
1104	10	0.19594912082769866
1104	11	0.05881520182135347
1104	12	0.07634118677653134
1104	13	0.055382232422552136
1104	14	0.0693472666383883
1104	15	0.04731444354040093
1104	16	0.09863310176647389
1104	17	0.050690370544643315
1104	18	0.12450582984099867
1104	19	-0.007126432899816848
1104	20	0.03494410912693979
1104	21	0.03730779842878462
1104	22	0.09252383558147415
1104	23	0.0019962072989798685
1104	24	-0.00992917508314406
1104	25	0.04196584666338765
1104	26	0.04724495507152682
1104	27	0.030188812344192684
1104	28	0.04922181994463415
1104	29	-0.03423583844897138
1104	30	0.07256839280354654
1104	31	0.1645287106175175
1104	32	0.1254199384714596
1104	33	0.12704470976910898
1104	34	0.12506890407431825
1104	35	0.18750142571515482
1104	36	0.186115367373354
1104	37	0.23866697191964192
1104	38	0.11536018078066235
1104	39	0.10067057974353588
1104	40	-0.01646557061034315
1104	41	0.14617443394457297
1104	42	0.21118019614983297
1104	43	0.09971259513824293
1104	44	0.0032116017991867807
1104	45	0.05147432466275603
1104	46	0.20346244524484547
1104	47	0.2196976459818174
1104	48	0.17734784109278934
1104	49	0.17788661534154926
1104	50	0.2028973430795986
1105	1	0.020260839935868802
1105	2	0.031509959709659086
1105	3	0.05776194301304827
1105	4	0.07444632556521924
1105	5	0.01820130899876915
1105	6	-0.08764590896958424
1105	7	0.004624229677240521
1105	8	-0.016895638120054457
1105	9	-0.03566350170399346
1105	10	0.04877161019056331
1105	11	0.034604921846714376
1105	12	0.08385582987256868
1105	13	0.015659582056819943
1105	14	0.13084948797730078
1105	15	0.05118298113423947
1105	16	0.06472543779651829
1105	17	0.06410530207159582
1105	18	0.10422049348343876
1105	19	0.16007357798658717
1105	20	-2.306533650653364E-4
1105	21	0.1048552082583214
1105	22	-0.0017268912498309419
1105	23	0.03661425956161679
1105	24	0.03175979009271401
1105	25	0.044437085681536814
1105	26	0.06670314308829717
1105	27	0.02706107105556299
1105	28	0.08153755725872003
1105	29	0.18503835589199724
1105	30	0.11707098113861036
1105	31	-0.025850185818583782
1105	32	0.06603796200608514
1105	33	-0.04155457369379434
1105	34	0.015453226912661933
1105	35	0.04006607697179021
1105	36	0.009813532736998809
1105	37	0.038352299389998734
1105	38	0.031153380087459936
1105	39	0.04643129539694968
1105	40	0.10515324949660965
1105	41	-0.07869206270041929
1105	42	-0.09242700893633327
1105	43	0.06106380121494226
1105	44	0.09255399660825248
1105	45	0.16532393956880043
1105	46	-0.05313842447471728
1105	47	0.16069278767181316
1105	48	0.11284950651947412
1105	49	0.02458466218114097
1105	50	0.11756183392136421
1106	1	-0.02307531391699058
1106	2	0.005264964064476629
1106	3	-0.06994297320612475
1106	4	0.07070584062807228
1106	5	0.05811294538507639
1106	6	0.0614101109734122
1106	7	0.023769092632846878
1106	8	0.1009319841082359
1106	9	0.08892205290020883
1106	10	0.047269633256696864
1106	11	0.03303204080670952
1106	12	0.004473402960783801
1106	13	0.054474467695037276
1106	14	0.020198015015736814
1106	15	0.05565111317478255
1106	16	0.005715369748685362
1106	17	-0.015332188667287028
1106	18	0.05730602897873638
1106	19	-0.04186559064851991
1106	20	0.016247092132445573
1106	21	-0.003632013246104293
1106	22	-0.015882569176895345
1106	23	-0.06318142598219367
1106	24	-0.0024913351537776407
1106	25	-0.027972349827912524
1106	26	0.009804022701509041
1106	27	0.09512473858470116
1106	28	0.03508187416889541
1106	29	0.04327900543395202
1106	30	0.009347361656644182
1106	31	0.03741220094437381
1106	32	0.1210856135883347
1106	33	0.10911841187529348
1106	34	0.12205822097548946
1106	35	0.10237159354591796
1106	36	0.1824426536400594
1106	37	0.26250909367958286
1106	38	0.07349955840642508
1106	39	0.04298817188836779
1106	40	0.04209309867704292
1106	41	-0.055330500704755536
1106	42	0.010717261307881643
1106	43	0.05885672233672068
1106	44	0.11024373297907886
1106	45	-0.05462420153673975
1106	46	0.05313593519247917
1106	47	-0.05598479677447443
1106	48	0.10731144325088593
1106	49	0.047664786838275575
1106	50	0.00970227299713855
1107	1	0.03867686697379824
1107	2	0.04354972722530093
1107	3	0.16752187035766752
1107	4	0.15792160977939318
1107	5	0.07505556690320248
1107	6	0.15348819401410907
1107	7	0.08725923294079507
1107	8	-0.04115364664193066
1107	9	0.0837697480680859
1107	10	0.07414543768362591
1107	11	0.04890010684519552
1107	12	0.13704296106225655
1107	13	0.12211783445015811
1107	14	0.022450410420368883
1107	15	0.07929530620546643
1107	16	0.0816671756374916
1107	17	0.12552197369586082
1107	18	0.11864705832741151
1107	19	0.10327487619716891
1107	20	0.04878566490030778
1107	21	0.04129076176859314
1107	22	0.05669461608361814
1107	23	0.09754704715590634
1107	24	0.13240013672901796
1107	25	0.08339013090557046
1107	26	0.09166489060864791
1107	27	0.09979041478001704
1107	28	0.10133685962962602
1107	29	0.22183199035442414
1107	30	0.018801860885986343
1107	31	0.0274690868388916
1107	32	0.046035560357118754
1107	33	0.09753267784068242
1107	34	0.03612754867319978
1107	35	0.04621067638004206
1107	36	0.017241169522030475
1107	37	-0.012078563034698675
1107	38	-0.0017431600401778269
1107	39	-0.029688325338838695
1107	40	0.014323486549855602
1107	41	0.2190823669481503
1107	42	0.12295765982693752
1107	43	0.18319133215986483
1107	44	0.12673547816840625
1107	45	0.05530421439766542
1107	46	0.1002654221071353
1107	47	0.16451160415985555
1107	48	0.11656334606816898
1107	49	0.11852387507497042
1107	50	-0.02218481326920596
1108	1	-0.014666910562059599
1108	2	-0.10038482201068177
1108	3	-0.20435490282518032
1108	4	-0.18836987429656205
1108	5	-0.1015617105153197
1108	6	-0.16992494240977862
1108	7	-0.16878100214222513
1108	8	-0.0853478280556601
1108	9	-0.1114087317013599
1108	10	-0.13399376198432122
1108	11	-0.2760953814397382
1108	12	-0.12271914882546753
1108	13	-0.12107921961558077
1108	14	-0.12826202453199897
1108	15	-0.12469285284706189
1108	16	-0.13897747783016712
1108	17	-0.17630438520403402
1108	18	-0.13518117734084711
1108	19	-0.21269236478823217
1108	20	-0.18804887464440867
1108	21	-0.1779829696884363
1108	22	-0.18069525526166785
1108	23	-0.2583749177123167
1108	24	-0.21870952183585501
1108	25	-0.15838752292989966
1108	26	-0.11320362765741265
1108	27	-0.19821308397235526
1108	28	-0.1639420015850277
1108	29	-0.13088450003968433
1108	30	-0.04538207572674935
1108	31	-0.21033821394595265
1108	32	-0.13197037500604125
1108	33	-0.1501962358088349
1108	34	-0.1877147404236652
1108	35	-0.18138983523815
1108	36	-0.10695696372054897
1108	37	-0.36935520292215074
1108	38	-0.09173686575425677
1108	39	-0.19199892253313777
1108	40	-0.10588226604998141
1108	41	-0.29509256375142456
1108	42	-0.24912677705737707
1108	43	-0.2651656787913481
1108	44	-0.2402537971093608
1108	45	-0.12788591258154725
1108	46	-0.18784256406864586
1108	47	-0.21914590190419148
1108	48	-0.26971855272078216
1108	49	-0.11161002859928715
1108	50	-0.2108286304766267
1109	1	-0.05317270363786619
1109	2	-0.036842055703932175
1109	3	-0.08270320513162943
1109	4	-0.1019971779784754
1109	5	-0.03928899458911417
1109	6	-0.06367158244028559
1109	7	-0.0817145603056062
1109	8	-0.05559853152383454
1109	9	-0.0630025601383366
1109	10	-0.019264415440957805
1109	11	-0.06154414493478555
1109	12	-0.0411394512534784
1109	13	-0.11876409662351506
1109	14	-0.16793842555335203
1109	15	-0.17126456246466404
1109	16	-0.13636972546254356
1109	17	-0.27810359234167237
1109	18	-0.20295836442322468
1109	19	-0.1264999672370024
1109	20	-0.1398561041049149
1109	21	-0.16016916210407572
1109	22	-0.08849335103173672
1109	23	-0.082219133427607
1109	24	-0.11288671731137612
1109	25	-0.18850374802813974
1109	26	-0.05680927558977089
1109	27	-0.1250603074708323
1109	28	-0.35259840992868163
1109	29	-0.2368749412656328
1109	30	-0.28013848653046125
1109	31	-0.24868686174589527
1109	32	-0.3620104830026065
1109	33	-0.372220578086889
1109	34	-0.2678420990951196
1109	35	-0.272596636793196
1109	36	-0.4074703742948955
1109	37	-0.3803625624763285
1109	38	-0.3867112106009074
1109	39	-0.31360078595503893
1109	40	-0.3450680482146993
1109	41	-0.2785326171925817
1109	42	-0.4090091844560746
1109	43	-0.4816370270697803
1109	44	-0.48923929434143076
1109	45	-0.39907226128325135
1109	46	-0.35344415507525184
1109	47	-0.44797877240888795
1109	48	-0.46004617280961924
1109	49	-0.21497150849808122
1109	50	-0.05862553963029171
1110	1	-0.12552900679621715
1110	2	-0.04511073650719055
1110	3	0.047557030212132645
1110	4	0.022185398770560028
1110	5	-0.060059599999114255
1110	6	0.01291564786528454
1110	7	-0.06661388198865126
1110	8	0.01970651864424866
1110	9	-0.020332603106965053
1110	10	-0.051823058921101506
1110	11	0.007617132833011908
1110	12	-0.06192520403132562
1110	13	-0.06448359303234241
1110	14	0.07847040083571244
1110	15	-0.10410042059690738
1110	16	-0.0858776676730173
1110	17	-0.12549011895399786
1110	18	-0.11292365734837517
1110	19	-0.04706253691489337
1110	20	0.04247810884498193
1110	21	-0.10480642363104664
1110	22	0.025220256080466186
1110	23	0.10627881080726809
1110	24	0.1479333148509383
1110	25	0.044858561328865676
1110	26	-0.028550524331649285
1110	27	-0.08451472422945017
1110	28	0.00464691470258316
1110	29	0.04157038992354245
1110	30	-0.020217138792510525
1110	31	0.12865777259307976
1110	32	0.07297006421298846
1110	33	0.13381377685621487
1110	34	0.17564503170115842
1110	35	0.029287731408302875
1110	36	0.12182107104199957
1110	37	0.06286599384051783
1110	38	0.057577661465494145
1110	39	0.11925289260997829
1110	40	0.07362907856128231
1110	41	0.07077940718006914
1110	42	0.06630648668993899
1110	43	0.11536938971165256
1110	44	0.16837067198507327
1110	45	0.08366187781876988
1110	46	0.20549104555431086
1110	47	-0.0036318057316719887
1110	48	0.029327681336553724
1110	49	0.12543849689081554
1110	50	0.1207798851945725
1111	1	-0.12715852779111358
1111	2	0.05127123958719082
1111	3	0.09727398865272363
1111	4	-0.09871589786813928
1111	5	0.037936303587819964
1111	6	0.0059474964243122486
1111	7	0.11857143314238527
1111	8	0.032681092269164935
1111	9	0.05616190739545601
1111	10	-0.08540045005798902
1111	11	0.07598742861446084
1111	12	-0.08192825645940616
1111	13	-0.01791437795856865
1111	14	0.039201576262222204
1111	15	0.10574913719085544
1111	16	0.06631832627826384
1111	17	0.11897818354903222
1111	18	0.021683158337602038
1111	19	0.16259648776610902
1111	20	0.039442736154427394
1111	21	0.11015078584933491
1111	22	-0.018720186876358014
1111	23	0.08761679958072932
1111	24	0.024061163637632887
1111	25	0.007286797107305176
1111	26	0.05228599689831981
1111	27	0.08312176082358746
1111	28	0.00100115385466637
1111	29	-0.08498247926139157
1111	30	0.041927841015848344
1111	31	-0.05264368840114031
1111	32	-0.02649045908155756
1111	33	0.017950253527750704
1111	34	-0.019963857530790108
1111	35	0.09312947919042358
1111	36	0.08597463039534858
1111	37	0.08212533366620534
1111	38	0.0640154389080081
1111	39	-0.001450464008357244
1111	40	0.04946256100748769
1111	41	0.036177133101652714
1111	42	0.10834920041155272
1111	43	0.07847584538263419
1111	44	0.10170825454779475
1111	45	0.27947396987812567
1111	46	0.1812850569477007
1111	47	0.10786729120962235
1111	48	0.1555370061729846
1111	49	-0.15756368892837003
1111	50	-0.30366813406028825
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	0.0
17	1.0
18	2.0
19	1.0
20	1.0
21	8.0
22	23.0
23	70.0
24	224.0
25	489.0
26	983.0
27	1685.0
28	2759.0
29	4769.0
30	7392.0
31	9660.0
32	10586.0
33	12147.0
34	14152.0
35	16778.0
36	20144.0
37	25699.0
38	37335.0
39	76965.0
40	258125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.25227490544843717	0.1729237301235611	99.46020590084194	0.11459546358605481
2	0.13060000000000002	0.26220000000000004	0.2042	99.40299999999999
3	99.35150142821709	0.1852281546795113	0.1856282154887543	0.2776422016146454
4	0.062200000000000005	99.45819999999999	0.30119999999999997	0.1784
5	0.0146	99.3052	0.40959999999999996	0.2706
6	0.0212	0.09079999999999999	99.6372	0.25079999999999997
7	0.173	0.0154	0.6185999999999999	99.193
8	0.2762	0.021	99.5906	0.1122
9	0.49699999999999994	0.019799999999999998	99.45580000000001	0.0274
10	99.43253984922403	0.05780612744950965	0.4870516274725121	0.022602395853960518
11	0.768	0.1898	99.0196	0.0226
12	99.3368	0.5	0.1478	0.0154
13	99.0472	0.9166000000000001	0.0246	0.0116
14	0.35660000000000003	99.5592	0.0558	0.0284
15	0.4724	99.4636	0.049	0.015
16	0.8618000000000001	99.0616	0.0504	0.0262
17	99.2204	0.5741999999999999	0.025	0.1804
18	98.98599999999999	0.6766	0.024399999999999998	0.313
19	0.5706	98.6928	0.051199999999999996	0.6854
20	0.7052	0.7704	0.037599999999999995	98.4868
21	98.434	1.2411999999999999	0.025799999999999997	0.299
22	0.32139999999999996	99.41799999999999	0.0526	0.208
23	0.0302	99.5886	0.0474	0.3338
24	0.0232	98.9906	0.0386	0.9476
25	0.0292	1.1468	0.030600000000000002	98.79339999999999
26	0.17520000000000002	98.3652	0.0288	1.4307999999999998
27	0.33080000000000004	0.45139999999999997	0.0634	99.1544
28	0.8076	0.21059999999999998	0.1874	98.79440000000001
29	89.056	8.433	1.1202	1.3908
30	21.8306	21.2182	25.546999999999997	31.4042
31	23.1254	22.8838	23.6202	30.370599999999996
32	20.4628	24.4032	25.748199999999997	29.3858
33	23.8288	22.192600000000002	23.0696	30.909
34	23.9764	23.6376	23.0778	29.3082
35	20.6788	24.4336	25.880599999999998	29.006999999999998
36	22.8432	21.921	23.3782	31.8576
37	23.008200000000002	24.0796	24.262	28.650199999999998
38	19.9524	24.665200000000002	26.8042	28.5782
39	22.212799999999998	23.0734	23.6142	31.0996
40	22.7346	23.1158	23.9776	30.171999999999997
41	19.939154037095637	24.08736056133501	26.961242201735768	29.01224319983358
42	22.53131543841614	21.492900900612607	24.82434754086557	31.15143612010568
43	22.096799999999998	22.8142	24.9602	30.1288
44	19.032568448552816	23.586270081476133	26.99614523675688	30.385016233214174
45	21.1362	21.4142	24.688	32.7616
46	20.6788	22.488	25.5812	31.252000000000002
47	17.170840391765484	23.810133341866784	29.34221079095107	29.676815475416657
48	20.305999999999997	20.7506	27.3668	31.5766
49	22.4122	15.939400000000001	32.742	28.906399999999998
50	89.1276	0.9402	9.035400000000001	0.8968
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	1.0
27	1.5
28	2.0
29	1.5
30	1.0
31	4.5
32	8.0
33	46.0
34	84.0
35	225.5
36	367.0
37	1056.5
38	1746.0
39	4141.5
40	6537.0
41	12482.5
42	18428.0
43	27224.5
44	36021.0
45	47325.5
46	58630.0
47	67155.5
48	75681.0
49	79590.5
50	83500.0
51	80202.0
52	76904.0
53	68511.5
54	60119.0
55	51663.5
56	43208.0
57	33451.5
58	23695.0
59	17247.0
60	10799.0
61	7007.5
62	3216.0
63	2018.5
64	821.0
65	505.5
66	190.0
67	112.5
68	35.0
69	20.5
70	6.0
71	3.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.963
2	0.0
3	0.015200000000000002
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0106
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0098
42	0.0014
43	0.0
44	0.019799999999999998
45	0.0
46	0.0
47	0.0014
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	500000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.52904622867907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.58405451304659	17.525122243192634
2	12.53086936715604	7.901727191252024
3	7.756501418161337	7.336652753580706
4	5.256324807735469	6.629076314241769
5	3.8533591140463073	6.074636882123391
6	2.8667390772442634	5.423132933519709
7	2.220935847488661	4.901679230448036
8	1.7341088319881393	4.373983802345177
9	1.3158856484360668	3.733975749707647
>10	6.806748132562983	34.34056569720341
>50	0.06874453120074656	1.3578669751728056
>100	0.005092187496351561	0.26711808216893196
>500	6.365234370439451E-4	0.1344621450437148
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGAATCTTGTGGAAAGGACGAAACACCGCTGCCTAGCTGCGACGCCATG	670	0.134	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	4.0E-4	0.0	0.0	0.0	0.0
26	4.0E-4	0.0	0.0	0.0	0.0
27	4.0E-4	0.0	0.0	0.0	0.0
28	4.0E-4	0.0	0.0	0.0	0.0
29	4.0E-4	0.0	0.0	0.0	0.0
30	4.0E-4	0.0	0.0	0.0	0.0
31	4.0E-4	0.0	0.0	0.0	0.0
32	4.0E-4	0.0	0.0	0.0	0.0
33	4.0E-4	0.0	0.0	0.0	0.0
34	4.0E-4	0.0	2.0E-4	0.0	0.0
35	4.0E-4	0.0	0.0022	0.0	0.0
36	4.0E-4	0.0	0.0022	0.0	0.0
37	4.0E-4	0.0	0.0022	0.0	0.0
38	4.0E-4	0.0	0.0022	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAATC	47950	0.0	45.359646	1
TCGATCG	25	4.4561722E-5	43.9716	44
TCGTCTA	25	4.4561722E-5	43.9716	41
TTTCGCA	20	7.877217E-4	43.971596	41
AGCCAGG	20	7.877217E-4	43.971596	44
GGATATG	20	7.877217E-4	43.971596	39
TCATCGG	30	2.5360805E-6	43.971596	44
TACCGCA	20	7.877217E-4	43.971596	38
CGGAATA	35	1.4523721E-7	43.9672	28
CGGAAAT	20	7.881098E-4	43.9672	28
CCGGGGT	95	0.0	43.9672	27
CCGGGCT	150	0.0	43.9672	27
CGTATGA	55	1.8189894E-12	43.9672	28
GTGGGCT	20	7.881098E-4	43.9672	29
CGTTAGT	25	4.4588043E-5	43.9672	28
CATAGGA	25	4.4588043E-5	43.9672	31
GGGAACG	25	4.4588043E-5	43.9672	29
CGAATAG	25	4.4588043E-5	43.9672	28
CGGTGGG	20	7.881098E-4	43.9672	28
CGTAAAG	40	8.35098E-9	43.9672	28
>>END_MODULE
