##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ToxA_R2_S2_L005_R1_001_x.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	500000
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11579	32.0	32.0	32.0	32.0	32.0
2	31.68312	32.0	32.0	32.0	32.0	32.0
3	35.46403	37.0	37.0	37.0	32.0	37.0
4	35.9567	37.0	37.0	37.0	32.0	37.0
5	36.24052	37.0	37.0	37.0	37.0	37.0
6	39.986502	41.0	41.0	41.0	37.0	41.0
7	39.985986	41.0	41.0	41.0	37.0	41.0
8	40.09015	41.0	41.0	41.0	37.0	41.0
9	40.20615	41.0	41.0	41.0	41.0	41.0
10	39.95276	41.0	41.0	41.0	37.0	41.0
11	39.688004	41.0	41.0	41.0	37.0	41.0
12	40.073794	41.0	41.0	41.0	37.0	41.0
13	39.971184	41.0	41.0	41.0	37.0	41.0
14	40.073732	41.0	41.0	41.0	37.0	41.0
15	40.202054	41.0	41.0	41.0	37.0	41.0
16	40.118772	41.0	41.0	41.0	37.0	41.0
17	40.105736	41.0	41.0	41.0	37.0	41.0
18	39.85139	41.0	41.0	41.0	37.0	41.0
19	39.603886	41.0	41.0	41.0	37.0	41.0
20	40.07391	41.0	41.0	41.0	37.0	41.0
21	39.93037	41.0	41.0	41.0	37.0	41.0
22	39.952722	41.0	41.0	41.0	37.0	41.0
23	40.047964	41.0	41.0	41.0	37.0	41.0
24	40.04321	41.0	41.0	41.0	37.0	41.0
25	40.12384	41.0	41.0	41.0	37.0	41.0
26	40.026324	41.0	41.0	41.0	37.0	41.0
27	40.18178	41.0	41.0	41.0	37.0	41.0
28	39.86103	41.0	41.0	41.0	37.0	41.0
29	39.584428	41.0	41.0	41.0	37.0	41.0
30	38.686918	41.0	41.0	41.0	32.0	41.0
31	38.568148	41.0	41.0	41.0	32.0	41.0
32	38.442126	41.0	41.0	41.0	32.0	41.0
33	38.477612	41.0	41.0	41.0	32.0	41.0
34	38.504756	41.0	41.0	41.0	32.0	41.0
35	38.532674	41.0	41.0	41.0	32.0	41.0
36	38.49373	41.0	41.0	41.0	32.0	41.0
37	38.49069	41.0	41.0	41.0	32.0	41.0
38	38.470934	41.0	41.0	41.0	32.0	41.0
39	38.449394	41.0	41.0	41.0	32.0	41.0
40	38.449442	41.0	41.0	41.0	32.0	41.0
41	38.428348	41.0	41.0	41.0	32.0	41.0
42	38.399408	41.0	41.0	41.0	32.0	41.0
43	38.363846	41.0	41.0	41.0	32.0	41.0
44	38.331794	41.0	41.0	41.0	32.0	41.0
45	38.345178	41.0	41.0	41.0	32.0	41.0
46	38.306878	41.0	41.0	41.0	32.0	41.0
47	38.289468	41.0	41.0	41.0	32.0	41.0
48	38.445798	41.0	41.0	41.0	32.0	41.0
49	38.478018	41.0	41.0	41.0	32.0	41.0
50	39.101412	41.0	41.0	41.0	37.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.069528312498786
1101	2	-0.014084282581375618
1101	3	-0.07022207973556505
1101	4	-0.040589063716431895
1101	5	-0.030952803578024657
1101	6	-0.07584296410369262
1101	7	0.06502262184318397
1101	8	-0.04056958663275623
1101	9	-0.12885703508246849
1101	10	-0.12593009538914401
1101	11	-0.008375227324911805
1101	12	-0.08955079183547099
1101	13	-0.08354373319058084
1101	14	0.046220995806457665
1101	15	-0.0430522749653619
1101	16	-0.06701189910267402
1101	17	-0.028018252955412493
1101	18	-0.10935704873230634
1101	19	-0.05698286691727361
1101	20	0.013694778084236248
1101	21	-0.008738506566622561
1101	22	-0.0417846478934365
1101	23	-0.13219665948861348
1101	24	-0.02711522428177915
1101	25	0.0019038625786578223
1101	26	0.0031021730854590146
1101	27	0.012348279665452822
1101	28	-0.03568167667646094
1101	29	-0.016579502684756164
1101	30	-0.08396388288252865
1101	31	0.11766934544413488
1101	32	0.07153203709583522
1101	33	-0.0023058542030298668
1101	34	0.04577698189669377
1101	35	-0.03281879917618369
1101	36	-0.06704380682142386
1101	37	-0.08479104910995972
1101	38	0.006831852112647141
1101	39	-0.022771132382764847
1101	40	0.01964282280647467
1101	41	0.09906170747407117
1101	42	0.10351296916838493
1101	43	0.01968900865365697
1101	44	0.07836192173650858
1101	45	0.07626044878786331
1101	46	-0.020384033499389886
1101	47	-0.030067257889918153
1101	48	0.0011918265275880913
1101	49	0.11732772204361908
1101	50	-0.03628748706076124
1102	1	0.024254722429034814
1102	2	-0.09085784300940603
1102	3	-0.02534722346781848
1102	4	0.010354069621051565
1102	5	0.010268748269858463
1102	6	-0.0053988890021017255
1102	7	-0.07023992104326737
1102	8	0.046819922726058394
1102	9	0.030821558389312997
1102	10	-0.00885703622216738
1102	11	-0.0013990409112949465
1102	12	0.01326204191600766
1102	13	-0.08373572727788314
1102	14	0.04296100842751116
1102	15	-0.01800035681502976
1102	16	0.07972214003463307
1102	17	0.06326590762266449
1102	18	-0.07461334127832941
1102	19	-0.03355025939983136
1102	20	-0.0527381854347837
1102	21	-0.061099928514558144
1102	22	0.08540993028983479
1102	23	0.06299903454744538
1102	24	0.0780117701771772
1102	25	0.01388152505967355
1102	26	0.0065619787621642445
1102	27	0.010413593165012003
1102	28	0.05818357055805734
1102	29	-0.010192238573417
1102	30	-0.02196965414053409
1102	31	-0.12669218243157587
1102	32	-0.06316866729375903
1102	33	-0.16976682869640314
1102	34	-0.07656809046070379
1102	35	0.021403581135217564
1102	36	-0.022335967353761532
1102	37	-0.057788495107104154
1102	38	-0.07882459463051106
1102	39	-0.07855263709279114
1102	40	-0.09119072945428286
1102	41	0.08448008756759862
1102	42	0.04220997932691972
1102	43	-0.06773480685868094
1102	44	-0.02803771064596816
1102	45	-0.11667976611911968
1102	46	0.03709622767275533
1102	47	-0.06832764701197647
1102	48	-0.12305001500265433
1102	49	-0.016224628996198476
1102	50	0.03827603484507591
1103	1	0.07372780668276135
1103	2	0.04520181197687023
1103	3	-0.018189228473545427
1103	4	-0.010094420203188292
1103	5	-0.011124429676932834
1103	6	-0.06671500935933494
1103	7	-0.009246296179696856
1103	8	0.0652414704070523
1103	9	0.0477731075679344
1103	10	0.02968835093511757
1103	11	-0.05451451847548583
1103	12	-0.0052493631890584425
1103	13	-0.07779302700841839
1103	14	0.016671570553576487
1103	15	-0.006592644376027579
1103	16	0.05313412554970398
1103	17	0.08303893681986096
1103	18	0.0058834388306436836
1103	19	0.06295976013392135
1103	20	0.008323101853626724
1103	21	0.024883455403980292
1103	22	0.011273418115450795
1103	23	-0.0892924913802986
1103	24	0.02682014652678788
1103	25	-0.044408447218124536
1103	26	-0.0205865133246661
1103	27	0.00829463874341485
1103	28	-0.0588883703299814
1103	29	-0.02533896798944113
1103	30	-0.08235349833594796
1103	31	-0.15671714641857193
1103	32	-0.09561310168221837
1103	33	-0.021105542700908586
1103	34	0.09823968037485287
1103	35	0.02154881831464195
1103	36	-0.0659779296933749
1103	37	-0.031095539218050305
1103	38	-0.08207312367309783
1103	39	-0.011068978969042575
1103	40	0.05147122670089743
1103	41	-0.018373348940158962
1103	42	-0.09066669728439791
1103	43	0.017075732964642043
1103	44	-0.003444961946158287
1103	45	-0.08804890666625909
1103	46	0.045441805965609205
1103	47	0.0657733747121867
1103	48	0.012044911716138529
1103	49	0.0021312818229972663
1103	50	-0.07709495025734014
1104	1	0.014147521838850707
1104	2	-0.03817383135203656
1104	3	-0.08410104142431152
1104	4	-0.027060728428253356
1104	5	0.021932912300663077
1104	6	0.056303624396662144
1104	7	-0.1583197723689338
1104	8	-0.011520881941912364
1104	9	0.01923876920947265
1104	10	0.053114419099777876
1104	11	0.004520032517824291
1104	12	0.0050370429348234325
1104	13	-0.06770801646347024
1104	14	0.027008010959406192
1104	15	-0.05880847308780801
1104	16	-0.10792865814424601
1104	17	-0.043234832718674454
1104	18	-0.02687595398837317
1104	19	-0.04710511519262184
1104	20	-0.038755295313556815
1104	21	-0.1136243252957172
1104	22	-0.04242473586689499
1104	23	-0.031279412167307896
1104	24	0.006429722906091229
1104	25	-0.017182377317674025
1104	26	-0.006200811065532719
1104	27	-0.07215562639922979
1104	28	-0.10520132543364724
1104	29	-0.13880747633427148
1104	30	-0.19755307133045363
1104	31	-0.08331481524630391
1104	32	-0.10452172077074806
1104	33	-0.20875857470028336
1104	34	-0.0948727296892855
1104	35	-0.1027212800181303
1104	36	-0.2221646491281959
1104	37	-0.1461755593532672
1104	38	-0.09813668847465351
1104	39	-0.18152957209009202
1104	40	-0.1588905563074121
1104	41	-0.20570994556751288
1104	42	-0.06275116973299788
1104	43	-0.11360161751834852
1104	44	-0.18862947692172582
1104	45	-0.1887163631195108
1104	46	-0.02889551101653609
1104	47	-0.08211056137585615
1104	48	-0.08522403231316389
1104	49	-0.023274460185838564
1104	50	-0.09939218774009362
1105	1	-0.04410108876383134
1105	2	-0.03511979183879532
1105	3	-0.03046468913305489
1105	4	-0.06032410657727638
1105	5	-0.07314790015011141
1105	6	-0.04633116932914305
1105	7	-0.08123734034124652
1105	8	-0.10405793253704587
1105	9	-0.06625007124001314
1105	10	0.017097205548573413
1105	11	0.04242998792398822
1105	12	0.021355181641602883
1105	13	0.06411313399078722
1105	14	-0.04223236864280011
1105	15	-0.058201478401350926
1105	16	-0.05267134231565507
1105	17	-0.04388030622306616
1105	18	-0.05642560622305837
1105	19	-0.09013656659977443
1105	20	-0.030500028915341204
1105	21	-0.010253927501004512
1105	22	-0.017035995808988957
1105	23	-8.66239793296586E-4
1105	24	-0.046962236892902354
1105	25	-0.05197797506357915
1105	26	-0.02332735160486976
1105	27	-0.0828468446316677
1105	28	-0.010810358457490565
1105	29	0.04774303283408443
1105	30	0.14352733067959633
1105	31	-0.03602088144928217
1105	32	-0.018757982099465664
1105	33	0.06798004388935652
1105	34	0.047012884917968734
1105	35	0.019878820373449457
1105	36	0.009765559309130367
1105	37	-0.0028131901119223812
1105	38	-0.0129293327112876
1105	39	-0.016712315276222967
1105	40	0.04744118518149065
1105	41	0.0826413029488009
1105	42	0.0030441423683456037
1105	43	0.09403632384409377
1105	44	0.14359386384391115
1105	45	0.16197438366074834
1105	46	0.03580471472586311
1105	47	-0.045085965438104836
1105	48	-0.05298120947934848
1105	49	-0.024437491124196242
1105	50	-0.031018448181370673
1106	1	0.06546550454406841
1106	2	0.02243068276814597
1106	3	0.11544394987304685
1106	4	0.14966220102250816
1106	5	0.015376509844479358
1106	6	0.07380177489257278
1106	7	0.06807219049977675
1106	8	0.04488758576825802
1106	9	0.09336870927837992
1106	10	0.07203423196986591
1106	11	0.10653745008826121
1106	12	0.10216465820523268
1106	13	0.08337254926478721
1106	14	0.07762366814986166
1106	15	0.04311634137341969
1106	16	0.03941350471648519
1106	17	0.011638137303499718
1106	18	0.03242303535868274
1106	19	0.0058435264067355774
1106	20	-0.04508556200028124
1106	21	-0.01754054148151596
1106	22	0.029715004783000154
1106	23	-0.02466481300574941
1106	24	0.031840450950717525
1106	25	0.04863246864908177
1106	26	0.027062762964256137
1106	27	0.027201881973425657
1106	28	0.028493134444644852
1106	29	0.046842188196897894
1106	30	0.06938255364843826
1106	31	0.030954095996946762
1106	32	0.08349789267010266
1106	33	0.05922996297894656
1106	34	-0.023248445572711773
1106	35	-0.03546520223561345
1106	36	0.06313043758617454
1106	37	0.03629797201769236
1106	38	-0.035156853441208114
1106	39	-0.0033632364411673166
1106	40	0.052760117137189866
1106	41	-0.07998894594508954
1106	42	-0.05154964694905573
1106	43	-0.0648648825300242
1106	44	-0.062458891762169344
1106	45	-0.09745408041705872
1106	46	-0.20471709501062207
1106	47	-0.14962705342681915
1106	48	-0.11570721077931267
1106	49	-0.19610319866544756
1106	50	-0.05355646258611557
1107	1	0.020300578142038006
1107	2	0.03496210114844445
1107	3	0.08099105994943301
1107	4	-0.12034642937882722
1107	5	0.07972185702428192
1107	6	0.06897404564635679
1107	7	0.05564491691857398
1107	8	-0.031149338984604924
1107	9	-0.00807828562091828
1107	10	-0.058143236567623546
1107	11	-0.16246919639247182
1107	12	-0.07795868907921744
1107	13	0.03427571540537144
1107	14	-0.0941194270562633
1107	15	-0.014420381961379292
1107	16	0.04723583822822519
1107	17	0.040801707049304525
1107	18	0.08196935615366385
1107	19	0.0750125681674163
1107	20	9.556432803421444E-4
1107	21	-0.005123228480371722
1107	22	-0.06992433396056441
1107	23	0.028723517859504
1107	24	0.033533119144252055
1107	25	0.05865747727002457
1107	26	0.030456546931887374
1107	27	0.09642930029824726
1107	28	0.00875895584892561
1107	29	0.030790902557086497
1107	30	0.1506738735072517
1107	31	-0.015950905916369607
1107	32	-0.0521727727281629
1107	33	0.06328871022797244
1107	34	-0.003295260298990854
1107	35	0.07260620881924496
1107	36	0.09303285542242179
1107	37	0.045572785051930964
1107	38	0.062142001155123694
1107	39	0.049542195416172774
1107	40	0.014335351352663395
1107	41	-0.0813821663203953
1107	42	0.07790813815836373
1107	43	-0.009948548250434897
1107	44	0.02087560784161724
1107	45	0.01692679073023129
1107	46	0.004093479342898831
1107	47	0.08297972493703298
1107	48	0.01923570185820722
1107	49	-0.06549679476896841
1107	50	-0.01972112862615205
1108	1	-0.03122966574249375
1108	2	-0.016135766748455183
1108	3	-0.07487232555455137
1108	4	-0.06808196211906647
1108	5	-0.0628480063693857
1108	6	-0.04388832418195676
1108	7	-0.005368229758857979
1108	8	0.10907590443083848
1108	9	0.030514942392144917
1108	10	-0.11622601617415995
1108	11	-0.2214093857405217
1108	12	-0.03288634768166787
1108	13	-0.09064423385163423
1108	14	-0.0620461026151915
1108	15	-0.01251466440079696
1108	16	-0.009645262454192505
1108	17	-0.09788982531566859
1108	18	-0.0208219163778125
1108	19	-0.06180751039448751
1108	20	0.021330341942828568
1108	21	0.0010524059090215587
1108	22	-0.04292612464979584
1108	23	0.0035494457342934993
1108	24	-0.03864103864035684
1108	25	-0.006446925214916632
1108	26	-0.03855473971299972
1108	27	5.119901663661608E-4
1108	28	0.01530007031842473
1108	29	-0.09795271887733747
1108	30	-0.10405432107825874
1108	31	-0.1427398615651967
1108	32	-0.125823535300718
1108	33	-0.17648335420420835
1108	34	-0.1283791861097967
1108	35	-0.10046818840375948
1108	36	-0.1207452541155476
1108	37	-0.08922165279809491
1108	38	-0.09134086900562721
1108	39	-0.13226619675700846
1108	40	-0.07918594316472394
1108	41	-0.1170939754875846
1108	42	-0.14753596500817423
1108	43	-0.16764667638873476
1108	44	-0.09280419734444934
1108	45	-0.21060903325194857
1108	46	-0.1270983691460259
1108	47	-0.2273675219292599
1108	48	-0.15016757848867002
1108	49	-0.17018154780478056
1108	50	-0.2202359007925594
1109	1	0.01855869474650973
1109	2	0.039477859683564986
1109	3	0.04006315739318467
1109	4	0.023142804637487302
1109	5	-0.05135274940966639
1109	6	0.01319881217676766
1109	7	0.05972605223664118
1109	8	0.042904650924342036
1109	9	0.04744456547347653
1109	10	0.15371831125046498
1109	11	0.1148199922755424
1109	12	0.07075216887252367
1109	13	0.13810828269144082
1109	14	0.056710510452077756
1109	15	0.008636898878116028
1109	16	-0.03270749758442548
1109	17	0.04788727336272558
1109	18	-0.021851344286886842
1109	19	-0.0798758305077385
1109	20	0.07037351093534738
1109	21	0.02578481830042989
1109	22	0.04789415839909594
1109	23	0.029962543676248288
1109	24	0.02285295817896582
1109	25	0.0901495423766221
1109	26	-0.001401059398226323
1109	27	0.04916280697228359
1109	28	0.023953603901595955
1109	29	-0.0031345016043573537
1109	30	0.09686408632641275
1109	31	0.01577319840168201
1109	32	0.1323240412357265
1109	33	0.05471639769763925
1109	34	-0.0038358374938809447
1109	35	-0.0016874489875462473
1109	36	0.10212603770776241
1109	37	0.12835632319430346
1109	38	0.12190534235024586
1109	39	0.11336378587014195
1109	40	0.10930953036827162
1109	41	0.035502447678666726
1109	42	0.139297661005223
1109	43	0.08810274618930691
1109	44	0.02342867489141298
1109	45	0.15709818188476987
1109	46	0.13917929146954577
1109	47	0.21624990969957736
1109	48	0.1347119755839543
1109	49	0.20957880339421564
1109	50	0.16699054729029683
1110	1	0.036587165431534174
1110	2	-0.017203954057318782
1110	3	0.010379811892711643
1110	4	0.03278580359884131
1110	5	0.08169585470993468
1110	6	-0.010744313543668227
1110	7	-0.020185128350391324
1110	8	-0.04499918660934554
1110	9	-0.09459160552171397
1110	10	-0.03001321108709476
1110	11	0.051071454114264725
1110	12	0.06467180034449882
1110	13	0.14440485030041117
1110	14	0.03067676142332232
1110	15	0.06791595293584862
1110	16	0.03539307440387063
1110	17	0.04646516478432261
1110	18	0.06642878699241095
1110	19	0.09053277988483188
1110	20	0.03770778381498019
1110	21	0.04289926501989072
1110	22	0.013141665673167324
1110	23	0.03830302284486464
1110	24	0.03247415273339982
1110	25	-0.03377421096689659
1110	26	0.01606716382354989
1110	27	0.006588444510079228
1110	28	-0.007529443800997626
1110	29	0.061289008745816886
1110	30	-0.10394561193833596
1110	31	0.04427218722230464
1110	32	0.020761457765729574
1110	33	0.019450830504212036
1110	34	-0.09852822553349228
1110	35	-0.029065230045304702
1110	36	0.045123992705981664
1110	37	-0.05444679265544039
1110	38	-0.029959542449105925
1110	39	-0.014871285763625508
1110	40	-0.11245034524597486
1110	41	-0.045696157342469235
1110	42	-0.12671279085337517
1110	43	-0.1109940062539394
1110	44	-0.1394058812358594
1110	45	-0.027484047775175213
1110	46	0.022693692232024887
1110	47	0.07588429172704281
1110	48	0.07494500598977538
1110	49	0.09182519403106681
1110	50	0.04549320512170851
1111	1	-0.2472395518071906
1111	2	0.06950301401032988
1111	3	0.0563186086803924
1111	4	0.11055183154319792
1111	5	0.020430007034754283
1111	6	0.036642412407530855
1111	7	0.09613090654417533
1111	8	-0.07663260755089141
1111	9	0.02861534515435693
1111	10	0.01351707663636148
1111	11	0.12878845192472
1111	12	-0.07159770212927441
1111	13	-0.06084979386082523
1111	14	-0.09947462745790148
1111	15	0.09192108082042694
1111	16	0.015065976668203973
1111	17	-0.08007390972954198
1111	18	0.12324059355133699
1111	19	0.13510951441887187
1111	20	0.014693911752573285
1111	21	0.12176051320645342
1111	22	0.026661660919046426
1111	23	0.11476205117286042
1111	24	-0.11924382080239582
1111	25	-0.05943494015279782
1111	26	0.006819849538977962
1111	27	-0.05594846446341961
1111	28	0.08342183962693639
1111	29	0.1053402737296878
1111	30	0.13339219554445947
1111	31	0.3527669659622745
1111	32	0.1519423511075999
1111	33	0.31375420920667807
1111	34	0.23769822796932516
1111	35	0.16678872022401947
1111	36	0.18508872438087565
1111	37	0.2561051980899265
1111	38	0.23754180876747455
1111	39	0.29822937348631484
1111	40	0.14675734062539902
1111	41	0.24655899393410152
1111	42	0.11324337980075683
1111	43	0.3158867261484488
1111	44	0.24852105154293014
1111	45	0.3167323922854237
1111	46	0.09678579726381287
1111	47	0.16169870599599534
1111	48	0.28500062438747875
1111	49	0.07485512025354524
1111	50	0.28654677798735406
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	0.0
16	0.0
17	1.0
18	1.0
19	3.0
20	0.0
21	8.0
22	20.0
23	67.0
24	203.0
25	435.0
26	888.0
27	1504.0
28	2549.0
29	4339.0
30	7225.0
31	9266.0
32	9893.0
33	10856.0
34	12003.0
35	13826.0
36	16492.0
37	21086.0
38	30414.0
39	63952.0
40	294967.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.3937726601927598	0.12814206156892038	96.85697685952755	1.6211084187107654
2	1.6412	1.4134	0.155	96.7904
3	96.76219999999999	1.6878	0.14220000000000002	1.4078
4	1.583	96.84660000000001	1.4212	0.1492
5	0.12739999999999999	96.6134	1.8259999999999998	1.4332
6	0.0246	0.0546	98.1786	1.7422
7	0.12739999999999999	0.0286	3.1866	96.65740000000001
8	1.414	0.033	98.49080000000001	0.062200000000000005
9	1.9578000000000002	0.029	97.96820000000001	0.045
10	98.01117725185222	0.047005264589634034	1.9122141679868148	0.029603315571343988
11	3.372	0.1596	96.4344	0.034
12	98.31299999999999	1.5602	0.0912	0.0356
13	96.4048	3.5228	0.038400000000000004	0.034
14	0.2304	99.66460000000001	0.031599999999999996	0.07339999999999999
15	1.558	98.33579999999999	0.0392	0.067
16	3.7404	96.1644	0.0284	0.0668
17	98.2528	1.5350000000000001	0.045399999999999996	0.1668
18	95.963	2.5492	0.037399999999999996	1.4504
19	1.5764	95.6816	0.0286	2.7134
20	2.7614	1.7066000000000001	0.053399999999999996	95.4786
21	95.41760000000001	4.399	0.0388	0.1446
22	0.1412	99.6104	0.0392	0.2092
23	0.043800000000000006	98.4046	0.0354	1.5162
24	0.0348	96.91579999999999	0.0278	3.0216
25	0.043	2.9746	0.041800000000000004	96.9406
26	0.1676	95.38759999999999	0.0292	4.4156
27	1.4184	0.2476	0.0708	98.2632
28	3.0138000000000003	0.5194	0.47080000000000005	95.99600000000001
29	89.2002	7.021199999999999	1.8002	1.9784
30	21.9252	21.0608	26.323200000000003	30.690800000000003
31	22.3094	23.0582	23.6066	31.025799999999997
32	19.4558	24.8492	25.2162	30.4788
33	23.3522	22.328799999999998	22.4892	31.829800000000002
34	23.1376	23.2016	23.3452	30.315599999999996
35	20.846400000000003	25.1256	25.6874	28.3406
36	22.5934	22.020999999999997	23.712	31.6736
37	22.3366	24.37	24.0624	29.231
38	20.50809219421266	25.377809067417616	26.277230653535682	27.836868084834038
39	22.9964	23.219	23.4738	30.3108
40	24.592	23.16	22.8704	29.377599999999997
41	20.6368	22.59	28.3066	28.466599999999996
42	22.435	21.7796	25.332	30.453400000000002
43	22.5546	23.1556	23.767	30.5228
44	19.0154	23.1632	27.639200000000002	30.182199999999998
45	21.3662	21.7514	24.206	32.6764
46	20.2268	22.261400000000002	25.470599999999997	32.041199999999996
47	17.605	23.709600000000002	29.707800000000002	28.9776
48	21.1518	19.5888	27.3762	31.883200000000002
49	23.4196	15.1694	32.2312	29.1798
50	86.40480000000001	0.7568	11.8826	0.9558000000000001
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.5
24	1.0
25	1.5
26	2.0
27	3.0
28	4.0
29	5.0
30	6.0
31	9.5
32	13.0
33	48.0
34	83.0
35	184.5
36	286.0
37	828.0
38	1370.0
39	3361.5
40	5353.0
41	11420.0
42	17487.0
43	26787.5
44	36088.0
45	46252.5
46	56417.0
47	66753.5
48	77090.0
49	79147.0
50	81204.0
51	78758.5
52	76313.0
53	70622.0
54	64931.0
55	54090.0
56	43249.0
57	33261.0
58	23273.0
59	17793.5
60	12314.0
61	8060.5
62	3807.0
63	2198.5
64	590.0
65	332.0
66	74.0
67	47.0
68	20.0
69	22.0
70	24.0
71	12.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.2034
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0112
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0024000000000000002
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	500000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.5348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.76980568011959	12.848
2	7.607961176976473	2.9724
3	4.38397116940025	2.5692
4	3.1881565206708027	2.4912
5	2.389581669635727	2.334
6	1.9749370354444375	2.3148
7	1.5306017978172288	2.093
8	1.2705530642750373	1.9855999999999998
9	1.0893380019247703	1.9151999999999998
>10	9.171325019964371	37.236200000000004
>50	1.202981346110531	16.1632
>100	0.4033826811638716	12.0474
>500	0.013309580850584597	1.7724
>1k	0.0040952556463337226	1.2574
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGAATCTTGTGGAAAGGACGAAACACCGTCCGCCTGAAGTACTGCACCG	2154	0.4308	No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGGCAAGTACCTGTCAGACCGCG	1589	0.31779999999999997	No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGCTTCAACCAAGTGACGCTACG	1373	0.2746	No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGGCGAGGACGACGAGTTCTACG	1171	0.2342	No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGTCTTGAACTAGCTGCCACCCG	896	0.1792	No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGTCACCTGGGCGTGCGCAGCGG	861	0.1722	No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGCGCACACCTGCTTATATACGT	814	0.1628	No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGATATCCATGTAGCGACAAGAG	806	0.16119999999999998	No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGTGACCGTAGGGATGGCCACTG	753	0.15059999999999998	No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGAGCAAAGGTTATTGTTCAGTG	670	0.134	No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGGCCCTCTCCGAAGCCGCTCCG	648	0.1296	No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGGGCCGCCCATGGATGTAGTCG	618	0.12359999999999999	No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGATCCATACAACTGCTCCGCCG	599	0.1198	No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGCACTCACAGGCTGCCCAGCCG	581	0.11620000000000001	No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGGAAGAAGTCGGCCTCCTCTAG	568	0.1136	No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGAAGGGCTTGAGTGTCAGATGT	526	0.1052	No Hit
TCGAATCTTGTGGAAAGGACGAAACACCGCAACGGGTTCTCCCGGCTACG	522	0.1044	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0016	0.0	0.0
36	0.0	0.0	0.0016	0.0	0.0
37	0.0	0.0	0.0016	0.0	0.0
38	0.0	0.0	0.0016	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAATC	47600	0.0	44.462425	1
CTTAAGG	20	7.8628527E-4	43.9879	44
AAGTAAA	25	4.446433E-5	43.9879	32
CGGAAAA	50	2.7284841E-11	43.9879	28
GATACGG	20	7.8628527E-4	43.9879	36
CGAACCG	25	4.446433E-5	43.9879	28
CGTATAG	25	4.446433E-5	43.9879	28
CGAGTGG	40	8.3164196E-9	43.9879	28
ACGTTTA	20	7.8628527E-4	43.9879	30
CAACGGA	25	4.446433E-5	43.9879	30
CGCGGGT	30	2.5296195E-6	43.9879	28
CTATATT	20	7.8628527E-4	43.9879	33
GTTTAAA	20	7.8628527E-4	43.9879	29
GTGGGTA	20	7.8628527E-4	43.9879	29
TTAGAGT	20	7.8628527E-4	43.9879	38
TTAGAAC	35	1.447006E-7	43.9879	36
ATAGCGG	20	7.8628527E-4	43.9879	44
AAGACCG	20	7.8628527E-4	43.9879	44
GGATCGG	25	4.446433E-5	43.9879	44
ATTAAGC	25	4.446433E-5	43.9879	31
>>END_MODULE
